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KMID : 0859320060240030179
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Journal of the Korean Society for Therapeutic Radiology and Oncology
2006 Volume.24 No. 3 p.179 ~ p.184
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Effects of Recombinant Human Epidermal Growth Factor on the Proliferation and Radiation Survival of Human Fibroblast Cell Lines in Vitro
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Kim Hyun-Sook
Kang Ki-Mun
Lee Sang-Wook
Na Jae-Boem
Chai Gyu-Young
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Abstract
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Purpose: To explore the effect of recombinant human EGF on the proliferation and survival of human fibroblast cell lines following irradiation.
Materials and Methods: Fibroblast was originated human skin and primary cultured. The trypan blue stain assay and MTT assay were used to study the proliferative effects of EGF on human fibroblast cell lines in vitro. An incubation of fibroblasts with rhEGF for 24 hours immediately after irradiation was counted everyday. Cell cycle distributions were analyzed by FACS analysis.
Results: Number of fibroblast was significantly more increased rhEGF (1.0 nM, 10 nM, 100 nM, 1,000 nM) treated cell than control after 8 Gy irradiation. Most effective dose of rhEGF was at 160 nM. These survival differences were maintained at 1 week later. Proportion of S phase was significantly increased on rhEGF treated cells.
Conclusion: rhEGF cause increased fibroblast proliferation following irradiation. We expect that rhEGF was effective for radiation induced wound healing.
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KEYWORD
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Recombinant human epidermal growth factor, Radiation, Fibroblast cell
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